Antioxidant and anti-inflammatory activities from fruiting body extracts of Lyophyllum decastes
pp.101-109
Lyophyllum decastes has been used for culinary purpose. The present study was conducted to evaluate the antioxidant and anti-inflammatory effects from methanol, acetone, and hot water extracts of L. decastes fruiting bodies. The acetone and methanol extracts showed the higher 1,1-diphenyl-2-picryl-hydrazy radical scavenging activities than that of the hot water extract at 0.5–2.0 mg/mL and was comparable to the BHT, the positive control. The ferrous ion chelating effects of the mushroom extracts at 0.5–2.0 mg/mL were significantly higher than that of BHT. The reducing power of acetone extract (2.12) was significantly lower than that of BHT (2.73) at 2.0 mg/ mL. The mushroom extracts also showed inhibitory effects on production of nitric oxide (NO), and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide-induced murine macrophage cells in a concentration dependent manner. In vivo anti-inflammatory experiment on carrageenan-induced hind-paw edema of rat model, the acetone extract of the mushroom significantly suppressed the carrageenan-induced rat hind paw edema of rats in a dose dependently. The results suggest that the fruiting bodies of Lyophyllum decastes are a good natural resource of antioxidant and anti-inflammation.
MATERIALS AND METHODS
Mushrooms and extraction
Animals
Assay for the total phenol content
Assay for the total flavonoid content
Evaluation of antioxidant activity
Reducing Power
Cell viability assay
Evaluation of anti-inflammatory activity
Western blot analysis
Carrageenan-Induced hind paw edema in rats
Statistical analysis
RESULTS AND DISCUSSION
Evaluation of phenolic contents
Evaluation of flavonoid contents
Evaluation of Antioxidant Activities
Chelating effect on ferrous ions
Assay for cytotoxicity
Anti-inflammatory activity of fruiting body extractsof L. decastes
Western Blot analysis
Carrageenan-induced paw edema in rats