표고수확후배지 퇴비 물 추출물에서 Bacillus subtilis BSM 320의 고밀도 배양 및 표고 푸른곰팡이병의 생물학적 방제 High density culture of Bacillus subtilisBSM320 in aqueous extract of composted spent mushroom substrate of Lentinula edodes and biological control of green mold disease

김자윤, 박세현, 박성준, 황보주형, 강희완

언어KOR
URLhttps://db.koreascholar.com/Article/Detail/426176

한국버섯학회지

제21권 제3호 (2023.09)
pp.140-144

한국버섯학회 (The Korean Society of Mushroom Science)

초록

The objective of this study was to achieve biological control of green mold disease in Pyogo mushrooms using antagonistic microorganisms. Bacillus subtilis BSM320 cells inhibited mycelial growth by 48–60% against three Trichodermaisolates including T. hazianumisolated from the substrates of Lentinula edodes, showing their antifungal activity.The bacteria were cultured to a high density of 4.2 x 109±113.7 cfu/mlin aqueous extract of composted spent mushroom substrates of L. edodes containing 1% glucose and showed a higher growth rate than that observed when using the commercial medium, Luria-Bertani broth. The bacterial culture showed a 75% protective effect without damaging the mushroom fruiting bodies. These results suggest that B. subtilis BSM320culture is suitable for biological control of green mold disease during mushroom cultivation.

키워드

Bacillus subtilis BSM320 Biological control Green mold

서 론
재료 및 방법
    표고수확후배지 퇴비 추출 배지 제조 및 B. subtilisBSM320 배양
    항균활성검정
    표고 푸른곰팡이병 발병도
    통계처리
결과 및 고찰
    B. subtilis BSM320의 푸른곰팡이균 Trichoderma spp.에 대한 항균 할성
    LeCSMS 물 추출물을 이용한 B. subtilis BSM320의 고농도 배양
    B. subtilis BSM 320 배양액을 이용한 표고 푸른곰팡이병의 생물학적 방제
적 요
감사의 글

저자

김자윤(한경국립대학교 생명공학부) | Ja-Yoon Kim (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
박세현(한경국립대학교 생명공학부) | Se-Hyun Park (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
박성준(한경국립대학교 생명공학부) | Seong-Joon Park (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
황보주형(한경국립대학교 생명공학부) | Ju-Hyeong Hwang Bo (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
강희완(한경국립대학교 생명공학부, 한경국립대학교 유전공학연구소) | Hee-Wan Kang (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea) Corresponding author

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