KOREASCHOLAR

This study investigated the microscopic characteristics and genetic relationships of Ganoderma applanatum fruiting bodies. Basidiospores were brown, ellipsoid, and had one or two large vacuoles and a double wall. The surface of basidiospores was smooth or wrinkled and most had numerous small and shallow holes. The length and width of basidiospores of Ganoderma applanatum isolates GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823, and ASI 53399 were on average 7.6×4.8 mm, 7.9×4.6 mm, 7.7×4.9 mm, 8.2×5.3 mm, 7.7×5.0 mm, 8.0×4.9 mm, and 7.9×4.9 mm, respectively. In contrast, the basidiospores of Ganoderma lucidum isolate ASI 7125 were 7.7×5.2 mm. Using the universal ITS1/ITS4 primer set, the ITS region of the isolates were amplified and sequenced. The ITS sequences were very closely related to G. applantum isolate GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823 and ASI 53399, but were not the same species. Whereas, G. lucidum isolate ASI 7125 belongs to different group.

INTRODUCTION
MATERIALS AND METHODS
    1. Microscopical observation
    2. Randomly amplified polymorphic DNA (RAPD)analysis
RESULTS AND DISCUSSION
CONCLUSION
ACKNOWLEDGEMENTS
REFERENCES

• Woo-Sik Jo(Gyeongsangbuk-do Provincial Agricultural Research & Extension Service, Daegu 41404, Republic of Korea) Corresponding author
• Young-Hyun Rew(Gyeongsangbuk-do Provincial Agricultural Research & Extension Service, Daegu 41404, Republic of Korea)
• Seung-Chun Park(College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea)