양송이버섯 솜털곰팡이병균(Cladobotryum mycophilum)에 대한 길항미생물 Bacillus altitudinis HC7의 선발 및 적정 배양조건 Selection and appropriate culture conditions of antagonistic bacterium Bacillus altitudinis HC7 against button mushroom cobweb disease caused by Cladobotryum mycophilum
pp.60-66
This study was conducted to selection and investigate appropriate conditions for mass production of antagonistic microbes to control cobweb disease caused by Cladobotryum mycophilum. A grampositive bacterium was isolated from spent substrate of Agaricus bisporus and showed significant antagonistic activity against Cladobotryum mycophilum. The bacterium was identified as Bacillus altitudinis. based on the cultural, biochemical and physiological characteristics, and 16S rRNA sequence. The isolate is saprophytic, but not parasitic nor pathogenic to cultivated mushroom whereas it showed strong inhibitory effects against C. mycophilum cells in vitro. The control efficacy of B. altitudinis HC7 against cobweb disease of C. mycophilum was up to 78.2% on Agaricus bisporus. The suppressive bacterium may be useful for the development of biocontrol system. To define the appropriate conditions for the mass production of the Bacillus altitudinis HC7, we have investigated appropriate culture conditions and effects of various nutrient source on the bacterial growth. The appropriate initial pH and temperature were determined as pH 6.0 and 30oC, respectively. The appropriate concentration of medium elements for the growth of pathogen inhibitor bacterium(Bacillus altitudinis HC7) was determined as follows: 3.0% soluble startch, 10% soytone, 1.0% (NH4)2HPO4, 1.0 mmol KCl, and 0.5% L-asparagine.
재료 및 방법
병원성 곰팡이 분리
미생물 분리
길항미생물 HC7의 선발
DNA 분리 및 16S rRNA 유전자 염기서열 결정
생리·생화학적 특성
방제효과 검정
배양적온
초기 pH의 영향
영양원에 따른 길항미생물의 증식에 미치는 영향
결과 및 고찰
길항미생물 HC7의 선발
길항미생물 HC7의 16S ribosomal DNA 분석
생리·생화학적인 특성
생물 검정 효과
온도와 pH에 따른 영향
탄소원에 따른 영향
유기질소원에 따른 영향
무기질소원에 따른 영향
아미노산에 따른 영향
무기염류에 따른 영향
길항미생물 HC7의 적정 배양조건
적 요
감사의 말씀
REFERENCES