Black bear (Echeveria spp.) and Adromischus maculatus are ornamental succulents that change color in response to light and temperature. Colors of leaves play an important role in their ornamental value and market demand. However, there is a lack of quantitative research on flavonoids contained in succulent leaves. In this study, a colorimeter and ultra-performance liquid chromatography (UPLC) were used to analyze color changes of leaf surfaces and components of bioactive substances to understand the relationship between color change and internal bioactive substances of black bear and A. maculatus. The colorimeter analysis showed that red, green, and blue colors of black bear increased by 42.31%, 49.32%, and 21.58%, respectively, compared to their initial values, resulting in a clear change in leaf color from reddish-black to green. On the other hand, in A. maculatus, the red color decreased by 0.15% and the green color decreased by 10.44%, resulting in a more pronounced red spotted pattern and an increased area. UPLC analysis detected quercitrin in Black Bear leaf extracts and quercetin-3-O-sambubioside in A. maculatus. These substances are considered to play a key role in the concentration of anthocyanins and color changes in response to environmental changes such as light and temperature. Results of this study could be used as basic data to understand the correlation between leaf color change and bioactive substances in succulents in response to light and temperature.