KOREASCHOLAR

Mycoleptodonoides aitchisonii water extract regulates osteoblast differentiation by activating the AMPK signaling pathway

Eugene Cho, Hyoun Woo Kim, Yun Jin Lim, Deuk-Sil Oh, Hyun Suk Kim
  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/445109
PDF 원문보기
한국버섯학회지
제23권 제3호 (2025.09)
pp.125-132
한국버섯학회 (The Korean Society of Mushroom Science)
초록

Bone-related diseases (e.g., osteoporosis) represent a significant health challenge, prompting research for effective therapeutic agents, particularly from natural sources. The edible mushroom, Mycoleptodonoides aitchisonii has attracted interest due to its wide range of biological activities. Cytotoxicity assays revealed no significant toxicity of the M. aitchisonii water extract (MAWE) up to 50 μg/mL. MAWE significantly promoted dose-dependent osteoblast differentiation with ALP activity and mineralization increase by 109.17 % and 23 %, respectively, compared with the differentiation-only group. Moreover, MAWE significantly upregulated osteoblast-related gene expressions, including that of type I collagen (COL1A), osterix (Osx), and osteopontin (OPN). Furthermore, MAWE treatment significantly increased AMPK phosphorylation. This effect was further confirmed by demonstrating that the AMPK inhibitor compound C suppressed AMPK phosphorylation, and subsequent MAWE treatment restored it. In summary, these results demonstrate that MAWE possesses potent osteoblast differentiation-promoting efficacy, primarily through AMPK signaling pathway activation.

키워드
Mycoleptodonoides aitchisoniiOsteoblastALP activityMineralizationAMPK
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
    Preparation of mushroom extracts
    Cell culture and differentiation
    MTT assay
    Alkaline phosphatase (ALP) staining and activity
    Alizarin Red S (ARS) staining
    Western blot analysis
    Statistical analysis
RESULTS AND DISCUSSION
    Effect of different solvent extracts of M. aitchisonii on cell viability
    Enhancement of alkaline phosphatase activity by MAWE in MC3T3-E1 cells
    Effect of MAWE on mineralization of MC3T-E1 cells during differentiation
    Effect of MAWE on the expression of osteogenesis-related genes during differentiation
    MAWE-Induced AMPK Phosphorylation During Differentiation of MC3T3-E1 Cells
CONCLUSION
REFERENCES
저자
  • Eugene Cho(Jeollanamdo Forest Research Institute, Naju 58213, Republic of Korea)
  • Hyoun Woo Kim(Jeollanamdo Forest Research Institute, Naju 58213, Republic of Korea)
  • Yun Jin Lim(Jeollanamdo Forest Research Institute, Naju 58213, Republic of Korea)
  • Deuk-Sil Oh(Jeollanamdo Forest Research Institute, Naju 58213, Republic of Korea)
  • Hyun Suk Kim(Jeollanamdo Forest Research Institute, Naju 58213, Republic of Korea) Corresponding author