Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    Animal    Total RNA Extraction and cDNA Cloning of Vps26a, Vps26b, Vps29, and Vps35 Genes from Micro Pigs    Preparation of Protein Extracts    Western Blot Analysis    Recombinant DNA Constructs    Yeast Two-Hybrid Assay   RESULTS    Isolation and Characterization of the Retromer Components Vps26a, Vps29, and Vps35 from Micro Pigs    Retromer Complex Formations among Vps26a, Vps26b, Vps29, and Vps35    Micro Pig Vps35 Interacts with Micro Pig Vps26a, Vps26b, and Vps29 in Yeast Two-Hybrid System   DISCUSSION   REFERENCES

• Ekyune Kim(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Young-Hyun Kim(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Chun-Hun Ryu(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Jae-Woong Lee(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Sang-Hyun Kim(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Sang-Rae Lee(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Myeong-Su Kim(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Wanb-Jun Kim(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB))
• Jeong mook Lim(Department of Agricultural Biotechnology, Division of Animal Genetic Engineering, Seoul National University)
• Kyu-Tac Chang(National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB)) Corresponding author