The aim of this study was to examine more effective cryoprotectant for the cryopreservation of mouse preantral follicles. Enzymetically isolated preantral follicles from 12-day-old mice were cryopreserved by a slow freezing protocol with 1.5 M propanediol (PROH), dimethyl sulphoxide (DMSO) or glycerol (GLY) and then grown and matured in vitro for 11 days after thawing. The survival of preantral follicles immediately after freezing and thawing was not different among the PROH (68.2%), DMSO (72.4%) and GLY (72.1%). After grown and matured in vitro, the rates of survival and metaphase II oocytes were 54.9% and 36.6% for PROH which was significantly higher rates (p<0.05) compared with the rates obtained from DMSO (16.9% and 9.0%) and GLY (16.3% and 7.5%). The diameter of metaphase II oocytes from pre antral follicles frozen in PROH (67.4+-1.8 um) was significantly (p<0.05) smaller than that of the fresh preantral follicles (69.1+-2.3 um). The results from the present study revealed that PROH is more suitable cryoprotectant for the cryopreservation of mouse preantral follicles.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS   RESULTS   DISCUSSION   REFERENCES

• Dong-Hoon Kim(Animal Biotechnology Division, National Institute of Animal Science) Corresponding author
• Duk-Soo Chung(Mirae Laboratory)
• Hyun-Joo Lim(Animal Biotechnology Division, National Institute of Animal Science)
• Gi-Sun Im(Animal Biotechnology Division, National Institute of Animal Science)
• Hwi-Cheul Lee(Animal Biotechnology Division, National Institute of Animal Science)
• Hwan-Hoo Seong(Animal Biotechnology Division, National Institute of Animal Science)