Separation of Barley Seed Proteins by Disc Electrophoresis
춘파2조, 춘파6조 급 춘파6조대맥의 각:5품종 계 15품종의 종자단백질을 disc electrophoresis에 의하여 분리하고, 그 결과를 사진, densitometric tracing 급 모식도로 제시했다. 품종에 따라 18∼21 bands를 식별할 수 있었다. 품종은 각각 고유의 pattern을 보이고 있어, electrophoretic patter이 각품종의 유전적 차이를 반영하는 것이라고 생각된다. 추파대맥은 전부 희박한 B2 band를 갖이고 있고 춘파대맥은 농후한 B2 band를 갖이고 있었으며, 또 B2 band는 대파대맥에서, B3 band는 춘파대맥에서만 볼 수 있어, 춘파대맥과 추파대맥을 electrophoretic pattern에 의하여 구별할 수 있었다.
Fifteen samples of barley seed proteins representing winter 6-rowed, spring 6-rowed and spring 2-rowed group were separated by disc electrophoresis. Photographic, densitometric and diagrammatic representation were presented to make comparison possible. Eighteen to twenty one bands were detected in each gel. Electrophoretic spectrum of each variety was highly variety specific showing that the spectrum is the reflection of hereditary constituents of a organism. Related variety showed identical or nearly identical pectrum. All winter 6-rowed barley showed very faint B2 band, while spring 6-rowed and spring 2-rowed barley showed dense or less dense B2 band. All spring barley showed B3 band, while all winter barley showed B4 band.