논문 상세보기
pp.179-187
부화 및 착상에서 prostaglandins의 역할에 대한 연구는 많으나 배아단계에 따라 포배의 팽창과 부화에 미치는 영향에 대한 연구는 미미하다. 따라서 본 실험에서는 다양한 농도의 prostaglandins를 발생단계에 따라 처리하거나, indomethacin과 prostaglandins의 양 변화를 통해 팽창과 부화에 미치는 영향을 알아보고, 배아 단계별 PG 와 PG 의 생합성양 변화양상을 알아보았다. 100M이 상 농도의 PG 처리시 거의 모든
The effects of prostaglandins in hatching and implantation have been studied but the results were various, and those are not well known by the embryonic stage. The present study examined the effects of prostaglandin (PG ) and prostaglandin (PG ) on the expansion and hatching of mouse embryos by embryonic stage. Also we tried to measure the concentration of prostaglandins of morula, expanded, and hatching embryos. In early morula stage embryos, high concentration of PG (>100 μM) showed cytotoxicity but PG did not. The hatching was inhibited all groups but not gave negative effects on expansion. In 84 hr and 96 hr stage embryos, the hatching rate was decreased at all treatment groups but not inhibited the expansion. When combine prostaglandin with indomethacin, the hatching rate was increased significantly compared to the prostaglandin-treated groups, and as lower and lower the PG concentration, the hatching rate increased to the control level. The embryonic synthesis of PG increased dramatically but that of PG increased gradually. PG showed cytotoxicity at early stage embryos much than late stage embryos, but PG did not. Hatching was inhibited by the high PG concentration. It is suggested that the inhibition of hatching might be at resulted from cytotoxicity of PG on embryo. However, it is thought that the mechanisms of inhibition of hatching are different between PG and PG . In conclusion, it can be suggested that PG and PG concerned with the expansion and hatching, and their effects on hatching were different by the embryonic stage.
