돌고기 Pungtungia herzi 정자의 미세구조를 주사 및 투과 전자현미경적 방법으로 연구하였다. 정자는 구형의 두부, 짧은 중편 및 꼬리로 구성되어 있으며 비교적 그 구조가 단순하고 그 길이는 약 37.4이었다. 정자의 미세구조에서 핵에 대한 접선 방향으로의 편모 배열, 짧은 핵 그리고 미토콘드리아의 비대칭적 배열은 잉어과 정자의 공통된 특징과 마찬가지로 나타났으며, 대부분의 경골어류에서와 같이 첨체를 가지고 있지 않았다. 반면 돌고기 정자에서는

The fine structure of spermatozoa of Pungtungia herzi was examined with scanning and transmission electron microscopies. The spermatozoa of p. herzi are approximately 37.4 in length and a relatively simple cell with a spherical nucleus, a short midpiece and a tail. The acrosome is not present as in most teleost fishes. The ultrastructure of spermatozoa represents typical characteristics of cyprinid spermatozoa including the lateral insertion of flagellum, the organization of centriolar complex in shallow nuclear fossa, and the occurrence and asymmetrical arrangement of mitochondria. In the nuclear envelope and mitochondrion, however there were some morphological differences for their ultrastructure. The nuclear envelope is severely undulated and the shallow nuclear fossa contains two centrioles which are at the angle of some 130 each other. The most significant feature can be observed with the mitochondrion; five or more mitochondria, which are shown in primary spermatocyte, fuse to form a single one in the mature spermatozoon. The mitochondrial aspect is different from that of other cyprinid spermatozoa, where their mitochondria have a conventional aspect and never fuse to form a mitochondrial derivative. In terms of sperm evolution the fused mitochondria are regarded as the apomorphic character in comparison with the separate mitochondria. The single mitochondrion is not reported in cyprinid spermatozoon except the case of Rhodeus.

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