암컷 미꾸라지, Misgurnus mizolepis와 수컷 미꾸리, M. anguillicaudatus간 잡종과 잡종 3배체를 생산하였으며, 잡종화 및 잡종 3배체화가 망막, 시개 및 trunk kidney에 미치는 영향을 조직학적으로 조사하였다. 잡종은 일부 조직의 핵과 세포 크기에서 양친과 거의 유사하였다. 잡종 3배체는 잡종에 비해 적혈구 세포 및 핵 크기, 망막 내과립층 horizontal cell 핵 크기, 망막신경절 신경세포 층의 신경세포,

Diploid and triploid hybrid between female mud loach Misgurnus mizolepis and male cyprinid loach, M. andguillicaudatus were produced, using artificial propagation. The influence of hybridization and triploidization on characteristics of the some tissues of retina optic tectum and trunk kidney in loach was determined histologically. Diploid hybrid have almost size similarities in nucleus and the cell size of some tissues to both parents. In the triploid hybrid the nucleus and／or the cell size in erythrocyte, horizontal cell in inner nuclear layer of retina ganglion cell in ganglion cell layer of retina, neuronal cell in granular layer of optic tectum, and secondary proximal tubule cell of trunk kidney, are much larger than those of the corresponding cells in the diploid hybrid individuals. However, triploid hybrid showed fewer number of cells in outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than in diploid hybrid. Results of this study suggest that some characteristics in the triploid hybrid exhibiting larger cell and nucleus size with fewer number of cell than diploid hybrid can be useful criteria for the distinction between diploid hybrid and triploid hybrid, and the ploidy level in loach.

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