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Effect of Follicular Fluid on Attraction and Motility of Human Spermatozoa KCI 등재

사람 정자에 대한 유인능과 운동성에 미치는 난포액의 영향

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Development & Reproduction (발생과 생식)
한국발생생물학회 (The Korea Society Of Developmental Biology)
초록

사람 정자에 대한 유인능과 운동성에 미치는 난포액의 영향을 밝히기 위하여 난관 폐색으로 내원한 환자에서 채취한 난포액 sample A, 남성 배우자의 불임으로 내원한 환자에서 채취한 난포액 sample B, Sample A를 가열처리한 난포액 그리고 modified human tubal fluid(m-HTF) 중 어느 하나를 함유한 각각의 모세관을 1, 2 및 4시간 동안 배양하여 유인된 정자의 수와 운동성을 가진 정자의 비율을 조사하였다. 유인된 정자

Follicular fluid has pivotal effects on attraction and motility of spermatozoa for successful fertilization. The effect of samples of human follicu1ar fluid(hFF) on attraction and motility of spermatozoa was investigated. Capillary tubes loaded with one of these samples, hFF sample A collected from patients with tubal factor, hFF sample B collected from patients with male factor, m-HTF and heated hFF sample were used for assessment of attraction and motility of spermatozoa following culture of 1, 2, and 4 hrs. Number and motile rate of spermatozoa in the tubes loaded with hFF sample A were significantly(P<0.05) higher than those of m-HTF, hFF sample B and heated hFF. Although the fresh hFF tended to increase the attraction of spermatozoa as compared to inactivated hFF, there was no significant difference between treatments.

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