논문 상세보기
pp.97-104
대부분의 포유동물에서 수란관내로 배란된 난자는 정자에 의해 수정이 된 후 개체발생을 시작한다. 그러나 수정이 되지 못한 난자들은 난구세포와 함께 수란관내에서 퇴화하여 제거되는데, 그 기작에 대해서는 구체적으로 알려져 있지 않다. 따라서 본 연구는 포유동물의 수란관내 물질이 난자-난구 복합체에 미치는 영향을 알아보고자 사람의 난포액과 소의 수란관 조직 추출액을 생쥐의 난자-난구 복합체에 처리하고 난자의 생존율 및 난구세포의 세포자연사(apoptosis)를
In most mammals, mature oocyte-cumulus complexer(OCCs) ovulate into the oviduct where fertilization by sperm takes place. However, the complex that fail to fertilize eventually undergoes degeneration while they reside in the oviduct. Yet there is no blown mechanism how both oocyte and cumulus cells degenerate. Using human follicular fluid (hFF), bovine oviductal tissue extract (BOX) and mouse OCC, the present study aimed to find how the oviduct influence the viability of the oocyte and cumulus cells in vitro. There was no difference of oocyte maturation rate between the control and BOX-treated groups. However, there was a significant difference in the survival of cumulus cells between two groups. Cumulus cells cultured in the presence of hFF alone underwent initially expansion and then they formed monolayer in the culture dish. Even after 72 hr, they proliferated well and showed fibroblast-like morphology. Cumulus cells cultured in the presence of both hFF and BOX also expanded after 24 hr, however, after 72 hr culture, they eventually detached and degenerated. Cumulus cells cultured in the BOX alone gave a similar drastic result. When the cumulus cells cultured in the presence of BOX were stained with DAPI, their nuclei showed partial condensation and fragmentation. After detailed analysis of these cells by TUNEL assay, many nuclei of them exhibited well stained spots indicating the signs of apoptosis. Based upon these observations, it is suggested that BOX might possess a factor that leads mouse cumulus cells to undergo apoptosis in vitro.
