논문 상세보기
pp.27-33
생쥐 신생자 시기에 생식 세포의 사멸 기전을 알아보기 위하여 세포 사멸을 보이는 생식 세포에서 세포 사멸 관련 단백질인 caspase-3, caspase-activated DNase(CAD), Bax 및 Fasnas ligand의 발현을 조사하였다. 활성화된 caspase-3와 CAD의 면역화학적 염색 결과 일차 및 이차 난포내 TUNEL 양성을 보이는 세포 사멸 난자에서 발현되는 것을 관찰하였다. CAD 또한 TUNEL 양성을 보이는 세포 사멸 난자에
To investigate the mechanism of germ cell death in postnatal stage of mouse, the involvement of apoptotic executioners, caspase-3 and caspase-activated DNase(CAD), and apoptotic initiators, Bax Fas and Fas ligand, in the germ cell death has been studied. Immune-labels of active caspase-3 and CAD were located in TUNEL-positive, apoptotic, oocytes as well as normal oocytes of primary or secondary follicles. CAD immune-labels were also detected in the nucleus of TUNEL-positive oocytes. Most of oocytes showing positive immune-labeling of active caspase-3 or CAD had vacuoles in their cytoplasm, which is the morphological characteristic of oocyte during folliclar atresia. Bax immune-stains were detected in the atretic oocytes which showed the vacuole in their cytoplasm. Positive immune-labels for Fas ligand was localized in TUNEL-positive or atretic oocytes. Presence of immunoreactivity of active caspase-3 and CAD in TUNEL-positive germ cells implicate that active raspase-3 and CAD might play a role in germ cell apoptosis during early development of mouse ovarian follicle. Immunohistochemical localization of Bax and Fas ligand in TUNEL-positive oocytes suggests that these might be the most plausible modulator of oocyte apoptosis.
