논문 상세보기
pp.99-111
난소가 제거된 생쥐를 이용하여 자궁조직에서의 ADAM-8, 9, 10, 12, 15, 17, 그리고 ADAMTS-1의 유전자의 발현이 생식호르몬에 의하여 조절되는 지를 알아보았다. 암컷 생쥐의 난소를 제거하고, 2주후에 sesame oil, 17 -estradiol (), progesterone ( 혹은 이 둘 혼합액 ()을 피하 주사하였다. RT-PCR 방법을 이용하여 유전자 전사체의 발현을 조사한 결과 ADAM-8, 12, 그리고 17은 oil을 주
The present study aimed to investigate whether the expression of ADAM-8, 9, 10, 12, 15, 17 and ADAMTS-1 genes is controlled by ovarian steroid hormones. Ovariectomized mice were injected with 17 -estradiol (), progesterone (, or . Uterine tissues were processed for RT-PCR and immunoblotting. The results of RT-PCR showed that administration of increases the level of ADAM-8, 12 and ADAM17 expression compared to or control group. In contrast, administration of markedly stimulated the expression of ADAM-9, 10, 15 and ADAMTS-1, whereas did not. Immunoblotting analysis using anti-mouse ADAM polyclonal antibodies demonstrated that alone or treatment results in the strong expression of ADAM-8, 12 and ADAM17 proteins but alone or control group gave weak expression. In contrast, alone or plus treatment increased the expression level of ADAM-9, 10, 15 and DAMTS-1 proteins. alone or control group did not increase the expression. These results indicate that expression of ADAM-8, 12 and ADAM17 genes is upregulated by and that of ADAM-9, 10, 15 and ADAMTS-1 gene is upregulated by .
