This study was conducted to elucidate the changes of photosynthetic ability and cation content in barley cultivar seedlings cultured for 10 and 30 days with different NaCl concentrations containing 1/4 Hoagland solutions. At the highest NaCl concentration, the weight of dry matter and the shoot/root ratio (S/R ratio) were decreased. Thus, shoots were affected more than roots by NaCl treatment. The S/R ratio decreased more in 'Neulssalbori' than in 'Bunong' by the NaCl treatment. The. internal Na+ concentration increased greatly with the highest NaCl concentration, but K+ concentration in plants decreased with the highest NaCl treatment. The Ca2+ concentration had a small change with NaCl concentrations. Thus Na+ /K+ and Na+ /Ca2+ ratios increased with the highest concentration. The chlorophyll content (%/dry weight) of seedlings decreased at higher NaCl levels except for Bunong in 30 day old seedlings. The photosynthetic ability decreased only for Neulssalbori in the 10 days NaCl treatment. The stomatal conductance, and transpiration had decreased in the 10 day old seedlings, but not with 30 day old seedlings.