논문 상세보기

In Vitro Development of Interspecies Nuclear Transfer Embryos using Porcine Oocytes with Goat and Rabbit Somatic Cells

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/133933
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro‐matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM‐199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1 kV/cm for 30 μs in 0.3 M mannitol medium. The activated cloned embryos were cultured in porcine zygote medium‐3 (PZM‐3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos reconstructed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to the morular stage than those cultured in PZM‐3. These results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine‐porcine cloned embryos and that culture condition could be an important factor in iSCNT. The lower developmental potential of goat‐porcine and porcine‐bovine cloned embryos may be due to incompatibility between the porcine oocyte cytoplasm and goat and bovine somatic nuclei.

목차
ABSTRACT   INTRODUCTION   MATERIALS AND METHODS   RESULTS   DISCUSSION   REFERENCES
저자
  • Yan Shi Quan(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea)
  • Kenji Naruse(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea)
  • Su Min Choi(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea)
  • Myung Youn Kim(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea)
  • Rong Xun Han(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea)
  • Chang Sik Park(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea)
  • Dong Il Jin(Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea) Corresponding author