Tobacco seeds (Nicotiana tabacum L. cv KF109) were primed in the polyethylene glycol 6000(PEG) solution and then stored at 5 and 25~circC under 40, 60 and 80% relative humidity (RH) conditions for six months. The effect of storage temperature and humidity on mean germination time (T50 ), longevity and germination of the primed tobacco seeds were compared. Untreated seeds (control) stored at 5~circC showed high germinability throughout the entire storage period and humidity, and a decline in germinability showed after 6 months at 60% RH and after 3 months at 80% RH when stored at 25~circC , Primed seeds retained high germinability until 6 months at 60% RH and 3 months at 80% RH when stored at 5~circC but showed a significant decline in germinability after 3 months at 40% RH, and 1 months at 60% and 80% RH, respectively when stored at 25~circC , Primed seeds were completely lost viability when stored at 25~circC under 60% RH for 6 months and under 80% RH for 3 months.