This study conducted to elucidate the change of the cation content (Na+ , K+ , and Ca2+ ), the L-proline content, and the sugar and starch content in the stems, roots, and leaves of three cultivars of the 30 days old seedling soybeans (Glycine max L. cv. Danwonkong, Hwang-keumkong, and Kwangankong) after 100 mM NaCl stress containing 1/2 Hoaglands nutrient solution in the sand culture. The reduction of the dry matter weight after 100 mM NaCl treatment among cultivars was higher in the order of Kwangankong, Danwonkong, and Hwang-keumkong. The highest reduction of the dry matter weight was occurred at the roots among three parts of plant. The Na+ content increased with NaCl treatment in overall and specially greatly increased in roots and stems. The K+ and Ca2+ content decreased with NaCl treatment at the roots and stems. The K+ content, however, at the leaves increased in all three cultivars with the NaCl treatment. The L-proline content with NaCl stress increased greatly. The increment of the L-proline content at the stems and roots of Hwangkeumkong was lower than that of other two cultivars, K wangankong and Danwonkong. The sugar content decreased with NaCl treatment at the stems and leaves. The starch content also decreased at the stems and leaves with NaCl treatment.aCl treatment.