Vg은 난생 척추동물의 성숙한 암컷 혈청에 존재하는 성 특이 단백질로서 에 의해 합성이 유도된다. 본 연구는 뱀장어 Vg과 ER 유전자 발현에 대한 androgen과 성장호르몬(GH)의 영향을 조사하였다. 미성숙 뱀장어()에 , 뱀장어 recombinant GH(eGH, ) 또는 methyltestosterone(MT, )를 각각 단독 또는 eGH 또는 MT와 혼합하여 주사한 후 10일 후에 샘플을 채취하였다. 간 ER과 Vg mRNA는 RT-PCR을

Vitellogenin(Vg) is a sex specific serum protein present in sexually maturing female blood of oviparous vertebrates. Estrogen() is a main inducer of hepatic Vg synthesis. We investigated the effects of androgen and growth hormone(GH) on regulation of Vg and estrogen receptor(ER) genes in Japanese eel. Immature eels() were given a single injection of alone, or in combination with eel recombinant GH(eGH, ) or methyltestosterone(MT, ) and sacrificed 10 days after the hormone treatments. Expression levels of ER and Vg genes from the liver were determined by means of reverse transcription and polymerase chain reaction(RT-PCR). Administration of stimulated Vg gene expression in a dose dependent manner. Levels of Vg mRNA after the injection of with MT(5mg/kg) or eGH() were much higher than in that of alone(). Whereas, injection of either vehicle, eGH () or MT(5mg/kg) alone did not induce the expression of Vg gene in the liver. ER mRNA was detected from the fish treated with vehicle alone. injection() increased this ER expression but dose dependent response was not clear. Addition of MT(5mg/kg) or eGH() did not affect ER mRNA expression. This study confirms the necessity of as the primary factor for Vg gene expression and requirement of additional hormones such as MT or GH for the full expression of Vg mRNA, and suggests that the additive effect of MT or GH on Vg gene expression would be mediated by some unknown factors other than ER.

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