점망둑의 난모세포 성숙과정에서 생성되는 주요 성 스테로이드 호르몬, -스테로이드를 분석하고자 전구물질 ()를 성숙기 난모세포(난경 ) 배양초기에 첨가하여 24시간 배양하였다. 스테로이드 대사물질 분석과 동정은 thin layer chromatography와 gaschromatography-mass spectrometry로 이루어졌다. 로부터 생성된 주요 성 스테로이드 대사물질은 -hydroxy, -dihydroprogesterone ()와 -hydrox

To investigate the -steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes () were incubated with radiolabeled -hydroxyprogesterone () for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two -steroids, -hydroxy, -dihydroprogesterone () and -hydroxy, -dihydroprogesterone (), were converted from in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at than . This result showed that is a major maturation inducing steroid (MIS) in longchin goby, suggesting may play a role in regulating the oocyte maturation process.

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