제브라피쉬 배아 유래세포의 최적 성장조건을 확립하기 위해 3종류의 배지 DMEM, K-NAC, D-NAC 그룹에서의 세포 성장률을 비교하였다. 실험 결과, 접종밀도에 따른 성장률의 경우, DMEM에 비해 K-NAC 그룹에서 초기 접종 효율이 높게 나타났으며, 후기 성장률은 DMEM 그룹에서 높게 나타났다. K-NAC, DMEM 그룹 모두 FBS 농도에 의한 성장차는 보이지 않았으며, 0.1% embryo extract를 첨가한 배지에서 효과는 낮게 나타

To optimize the cell culture conditions of zebrafish embryonic cells, we compared the efficiency of three types of medium, DMEM, K-NAC and D-NAC. In this study, we showed that the cells grown in K-NAC have better plating efficiency than DMEM, especially in the case of low cell seeding density. However, cells grew slower in K-NAC than those in DMEM in confluent cultures. The effect of 0.1% zebrafish embryo extracts was minimal. The presence of 1% trout serum in culture medium significantly increased the growth rate of cells(p<0.05). No difference was found at cell seeding density(p<0.05). At cell seeding density, cells grew better in DMEM than K-NAC (p<0.1). The results suggest that supplementation of NAC and A2P in Keratinocyte SFM may improves plating efficiency when cells are plated at low population. No difference was found for cell growth in either medium with 5%, 10% or 15% FBS supplemented (p<0.05). Cells culture in D-NAC grew significantly better than those in DMEM(p<0.05). Our results clearly showed that the use of NAC and A2P in the culture medium has a positive effect on cell growth regardless of the amount of FBS added.

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