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Briliant Cresyl Blue 염색 방법과 극체 방출 여부에 따른 돼지 체외수정용 난포란 선별 방법이 배발달에 미치는 영향

Different Developmental Competence of Porcine Oocytes Selected by Brilliant Cresyl Blue Staining and Polar Body Extrusion

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  • URLhttps://db.koreascholar.com/Article/Detail/141302
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

The brilliant cresyl blue (BCB) has been used to select the developmental competent oocytes in pigs, goats and cows. Growing oocytes have a higher level of active glucose-6-phosphate dehydrogenase(G6PDH) compare to mature oocytes and are rarely stained compared to mature oocytes, because G6PDH converts BCB to colorless. First polar body extrusion regard as a guideline of meoisis completion. Selection of polar body extrude oocyte is more developmental competent to blastocyst than unselected. This study was conducted to compare the BCB test to the polar body extrusion on selection of developmental competent porcine oocytes for the production of blastocyst. Cumulus-Oocytes complex were exposed to 26uM BCB stain diluted in NCSU-23 for 90 min. There was no significant difference embryo development to blastocysts between BCB treated and not treated(19.58±1.99 vs 18.75±2.27 %), which means there was no detrimental effect of BCB exposure to oocytes. Normal fertilization is not differed among treatment groups from 70.0 to 78.4% development to blastocyst, beside polyspermy did not. To compare two different selection methods, BCB test and polar body extrusion, evaluate the developmental competent of IVP embryos. BCB+PB+(blue stained and polar body extruded, 20.71±0.45%) and BCB-PB+(colorless and polar body extruded, 20.04±1.29%) groups are significantly (p<0.05) higher developed than those of BCB+PB-(blue stained and no polar body, 13.24±0.73%) and BCB-PB-(colorless and no poladbody, 7.25±0.77%). These results showed that selection of polar body extruded oocytes method is more efficient than that of BCB test.

목차
ABSTRACT   서론   재료 및 방법    난소 및 난포란의 채취    BCB 염색    난포란의 체외성숙 및 난포란의 극체 확인    체외수정    체외배양    수정란의 관찰    통계분석   결과    BCB 처리가 체외발달에 미치는 영향    BCB 염색 방법과 극체 유무에 의한 난포란 선별 방법이 수정율에 미치는 영향    BCB 염색 방법과 극체 유무에 의한 난포란 선별 방법이 체외발달에 미치는 영향   고찰   인용문헌
저자
  • 김연수(진주산업대학교 동물소재공학과) | Yeon-Soo Kim
  • 김철욱(진주산업대학교 동물소재공학과) | Cheol-Wook Kim
  • 김인철(축산과학원 양돈과) | In-Cheol Kim
  • 곽대오(경상대학교 과학교육과) | Dae-O Kwack
  • 정기화(진주산업대학교 동물소재공학과) | Ki-Hwa Chung Corresponding author