Placenta is the main nutrition source for the fetus during pregnancy. Thus, it has a pivotal function in the pregnant process. Many functions of the placenta have been elucidated. An abnormal placenta is associated with a high rate of pregnancy failure in somatic cloned bovine. Differentially expressed genes (DEGs) were examined in a comparison between normal and cloned bovine placenta using annealing control primer (ACP)-based GeneFishing PCR. Using 120 ACPs, nearly 80 genes were identified and the fragments of 42 DEGs were sequenced. 38 of these genes were known genes and four were unknown. To determine the DEGs result, six target clones expressing on one-side of a normal and a clone placenta were selected. Through an analysis of the target genes using the real-time PCR, the expressing pattern was found to be somewhat different from the DEGs. Additionally, several genes appeared with the same expression pattern. Taken together, this suggests that the target genes would be essential for research into what influences the placental formative mechanisms during fetal development.

ABSTRACT   INTRODUCTION   MAERIALS AND METHODS    Source for Nuclear Transferred Somatic Cells and Nuclear Transfer    Generation of Placental Tissues    RNA Isolation    cDNA Synthesis and ACP RT-PCR Analysis    Cloning and Sequencing    Semi-Quantitative RT-PCR    Real-Time PCR    Statistical Analysis   RESULTS    Analysis of DEGs in Cloned Bovine Placenta    Semi-Quantitative RT-PCR    Real-Time PCR   DISCUSSION   REFERENCES

• Hee-Ja Park(Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Yeoung-Gye Ko(Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Seongsoo Hwang(Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Byoung-Chul Yang(Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Hwan-Hoo Seong(Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Seok-Doo Oh(Department of Animal Science & Biotechnology, Jinju National University)
• Sue-Yun Hwang(Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Kwan-Sik Min(Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA)
• Jong-Taek Yoon(Animal Biotechnology, The Graduate School of Bio & Information Technology, Hankyong National University, Animal Biotechnology Division, National Institute of Animal Science, RDA) Corresponding author