Effects of FBS(Feta1 Bovine Serum) and pFF(Porcine Follicular Fluid) on In Vitro Maturation and Development of Porcine Parthenogenetic and Nuclear Transfer Embryos
ABSTRACT INTRODUCTION MATERIALS AND METHODS Preparation of Porcine Follicular Fluid (pFF) Collection of Oocytes and In Vitro Maturation (IVM) Production of Parthenogenetic (PA) Embryos Brilliant Cresyl Blue Test (BCB) Cdc2 Kinase Assay Apoptosis Assays Preparation of Porcine Fetal Fibroblast Cells Production of Nuclear Transfer (NT) Embryos Statistical Analysis RESULTS Effect of FBS and pFF on In Vitro Maturation and In Vitro Development of Porcine PA and NT Embryos BCB Staining and Cdc2 Kinase Activity of the Oo- cytes Matured in Different Condition DISCUSSION REFERENCES
저자
Hyo-Jin Moon(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
Joo-Hyun Shim(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
In-Sun Hwang(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
Mi-Rung Park(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
Dong-Hoon Kim(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
Yeoung-Gyu Ko(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
Choon-Keun Park(College of Animal Science, Kangwon National University)
Gi-Sun Im(Division of Animal Biotechnology, National Institute of Animal Science, RDA)
Corresponding author