ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    Construction of pBCl/tPA and pBlue/Neor    Preparation of Goat and Pig Fetal Fibroblasts    Co-transfection    PCR-based Transgene Detection   RESULTS     The Recombinant pBCl/tPA and pBlue/NeoU Vectors    Estimation of G418 Resistance for Goat and Pig Fetal Fibroblast Cells    Efficiency of DNA Co-transfection into Goat and Pig Fetal Fibroblast Cells   DISCUSSTION   REFERENCES

• Baek-Chul Kim(Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
• Rong-Xun Han(Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
• Myung-Yoon Kim(Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
• Young-MIn Shin(Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
• Chang-Sik Park(Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
• Dong-Il Jin(Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) Corresponding author