Some organotin compounds such as butyltins and phenyltins are known to induce impo-sex in various marine animals and are considered to be endocrine disruptors. In this study, the effect of organotins on follicular steroidogenesis in amphibians was examined using ovarian follicles of Rana dybowskii and Rana catesbeiana. Isolated follicles were cultured for 6 or 18 h in the presence and absence of frog pituitary homogenate (FPH) or various steroid precursors, and the levels of product steroids in the culture media oassay. Among the butyltin compounds, tributyltin (TBT) strongly and dose-dependently inhibited the FPH-induced synthesis of pregnenolone () and progesterone () by the follicles. TBT also strongly suppressed the conversion of cholesterol to and partially suppressed the conversion of to . A high concentration of dibutyltin (DBT) also inhibited steroidogenesis by the follicles while monobutyltin and tetrabutyltin had negligible effects. The toxic effect of TBT or DBT was irreversible and a short time of exposure (30 min) was enough to suppress steroidogenesis. All the phenyltin compounds significantly inhibited FPH-induced synthesis by the follicles. The effective dose of 50% inhibition by diphenyltin was and those of monophenyltin and triphenyltin were and , respectively. However, none of the phenyltin compounds significantly suppressed the conversion of to -hydroxyprogesterone (-OHP) (by -hydroxylase), -OHP to androstenedione (AD) (by lyase), or AD to testosterone by the follicles. Taken together, the data show that among the steroidogenic enzymes, P450scc in the follicles is the most sensitive to organotin compounds and that an amphibian follicle culture system can be a useful screening model for endocrine disruptors.

• Kwon Hyuk-Bang
• Kim Seung-Chang
• Kim An-Na
• Lee Sung-Ho
• Ahn Ryun-Sup