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Effects of 3,5,3'-Triiodothyronine (T3) on Osmoregulation following Freshwater Acclimation in Starry Flounder KCI 등재

강도다리 Platichthys stellatus 담수 순화시 삼투압 조절에 미치는 갑상선호르몬의 영향

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Development & Reproduction (발생과 생식)
한국발생생물학회 (The Korea Society Of Developmental Biology)
초록

본 연구에서는 외인성 T3처리가 강도다리의 담수 순화시 삼투압 조절과 혈중 코티졸 변화에 어떠한 영향을 미치는지 조사하였다. 해수에서 사육 중인 강도다리에 갑상선호르몬의 일종인 T3를 5, 10 및 /g body weight의 농도로 0.9% NaCl과 함께 주입하고 담수로 옮긴 후 3일째 혈액을 채취하였으며, 삼투압 조절 지표인 및 삼투질 농도와 스트레스 지표인 cortisol, glucose를 조사하였다. /g의 T3를 주입한 실험구의 혈장 와 의

The aim of this study was to test if 3,5,3'-triiodothyronine (T3) are involved in the osmoregulatory actions in euryhaline starry flounder Platichthys stellatus. We investigated osmoregulatory parameters ( and osmolality), blood cortisol and glucose in starry flounder acclimated to seawater (SW, 33 psu) and that were transferred and allowed to acclimate to freshwater (FW, 0 psu). Fish in SW were injected with T3 (5, 10, and /g body weight) or vehicle (0.9% NaCl), and then transferred to FW. They were sampled 3 days after the transfer. With T3 at /g, levels of plasma and were significantly higher than in sham (only saline) and control fish (without hormone and saline). Osmolality was significantly higher after injection with T3 at 10 and /g than in the control. However, T3 at /g had no effect on hyper-osmoregulation. In this study, all dose of T3 induced a significant increases in plasma cortisol without glucose. These results suggest a positive hyper-osmoregulatory role of T3 in starry flounder to hypoosmotic environment, maybe a positive interaction of T3 with cortisol for maintenance of hyper-osmoregulatory ability.

저자
  • 민병화
  • 임한규
  • 장영진
  • 김영수
  • 명정인