이 연구는 2006년 1월부터 3월까지 전남 여수시 거문도 해역에서 어획된 찰가자미의 어미로부터 실험실 내 자연 산란을 유도하여 난발생 및 자치어 형태 발달을 관찰하였다. 수정란은 무색 투명한 분리부성란이고, 유구는 없었으며, 난경은 1.640.03 mm(n=50)였다. 부화에 소요되는 시간은 수온 (평균 )에서 수정 후 168시간 40분이었으며, 부화 직후의 평균 전장은 4.050.18 mm(n=20)였다. 부화 직후 자어는 막지느러미가 발달하였고,
The slime flounder Microstomus achne were caught at Geomun Island, Yosu-si, Jeollanamdo from January to March in 2006. The fertilized eggs were observed for morphological development of egg, embryo and larva. Eggs were colorless transparent, separative pelagic, absent of oil globule, and the diameter was 1.640.03 mm (n=50). The eggs were hatched at 168 hours 40 minutes after fertilization in the range of (mean ). Total length of newly hatched larva was 4.050.18 mm (n=20). The larva had developed membranous fin showing waterdrop-shaped structure, and their mouth and anus were not open. The myotomes were 14~15+33~34=47~49. The egg yolks were 1.640.12 mm in major axis, and 1.230.19 mm in minor axis. At 12 days after hatching, the total length was 7.320.42 mm(n=20). The egg yolk was completely absorbed and transferred to post larval stage. Star-shaped melanophores and branch-shaped xanthophores in the edge of membranous fin were more densed. Chrysanthemum-shaped melanophores in the notochord were densed and formed 4~5 melanophore bands. At 90~93 days after hatching, morphological features of the larva, 19.911.63 mm TL(n=20), were transferred to juvenile stage showing similar features with those of the adult fish.