논문 상세보기
pp.113-120
정상 발정주기를 보이는 7주령부터 38주령의 생쥐를 대상으로 발정주기에 따른 자궁조직내 비만세포의 분포를 toluidine blue 염색법으로 조사하였다. 비만세포의 밀도는 생쥐의 연령 증가와 더불어 지속적으로 증가하다가, 30주령 이후 감소하는 경향을 보였다. 발정주기별 분포에서는 조사한 모든 연령의 생쥐에서 발정후기에 가장 높은 밀도를 나타내었고, 그 대부분은 자궁근층에서 발견되었다. 10주령의 생쥐를 대상으로 Alcian blue-safranin
We examined the distribution of uterine mast cells in cycling mice of different ages between 7 weeks and 38 weeks by toluidine blue staining. Mast cell density increased gradually depending on the age and culminated at 30 weeks after birth. Of the estrous stages, uteruses at metestrus showed markedly higher density of mast cells than those at the other stages in cycling mice of all ages analysed in this study and the majority of the mast cells were found in myometrium. We also discriminated types of the uterine mast cells in cycling mice at 10 weeks old by Alcian blue-safranin double staining. Mucosal type was most abundant among three types, including connective-tissue types and mixed types, through the entire estrous cycles. The portion of mixed types increased slightly after estrous. The abundance of uterine collagen protein determined by Masson trichrome staining was exactly consistent with the density distribution of the uterine mast cells during estrous cycles. Taken together, these results may imply that uterine mucosal mast cells, together with collagen proteins, are heavily involved in tissue remodeling of cycling mouse uterus.
