논문 상세보기

The Effects of Glucose on Blastulation and Cell Counts of Blastocysts in Mice

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/1714
서비스가 종료되어 열람이 제한될 수 있습니다.
한국발생생물학회 (The Korea Society Of Developmental Biology)
초록

The aim of this study was to investigate the effect of glucose on embryonic development of mouse embryos. Two cell embryos were recovered from ICR female mice(3-4weeks) at 46~50 hrs after hCG 5 IU injection (mated just after hCG injection) and cultured in 50 DMEM droplets supplemented with nothing (control: n=46), glucose 0.5mM (Group A; n=46) or glucose 3.15 mM(Group B; n=46) under mineral oil. All experimental media were supplemented with 20% human follicular fluid. Total blastocyst formation rates was lower (NS) in glucose groups (group A: 52.2% : B. 47.8%) than control group (60.9%). ZiB rates was the highest (P<0.05) in control (47.8%) than those in group A (21.7%) and B (28.3%). ZeB rates were the highest (NS) in group A (30.4%) than those in control (13.0%) and group B (19.6%). Blastocysts, cultured in group B (50.5), had the highest (NS) mean cell number compared with the others (control: 39.2 ; group A: (45.6). The ICM proportion (% ICM of total cells) in blastocysts cultured in group A (20.6%) was the highest (NS) than those of other tested groups (control: 15.2 ; group B: 13.9%). This study shows that a low dose of glucose added to culture medium increases the ICM proportion of blastocysts in mice.

저자
  • Park Sung-Baek
  • Kim Ji-Chul
  • Park Kee-Sang
  • Lee Taek-Hoo
  • Chun Sang-Sik
  • Song Hai-Bum