The objective of this study was to evaluated the efficiency on sperm cryosurvival and ability of in vitro fertilization using Triladyl and Lactose Egg-Yolk(LEY) as extenders for cryopreservation of separated sperm by 65% percoll in miniature pig. Sperm viability was measured with SYBR-14/PI double stained sperm by flow cytometry. Ability on embryo cleavage rate and blastocyst development were observed by in vitro fertilization after frozen-thawing of sperm separated by 65% percoll. The experimental groups were designed that separated sperm by 65% percoll with Triladyl (ST) or LEY(SL) and unseparated sperm with Triladyl(UT) or LEY(UL) for cryopreservation. As a results, the viability was significantly(p<0.05) higher in ST(55.1%), SL(63.1%), UL(58.8%) than UT(38.2%) group. Sperm viability in SL(63.1%) group was significantly(p<0.05) higher than other experimental groups. On the other hand, embryo cleavage rate was significantly(p<0.05) higher in ST(79.1%), SL(83.2) than UT(74.1%) and UL(75.7%) groups at 96h after in vitro fertilization. Blastocyst development was also significantly(p<0.05) higher in ST(21.5%), SL(20.9%) than UT(17.0%) and UL (18.8%) groups. In conclusion, cryopreservation of miniature boar sperm separated by 65% percoll were beneficial to viability and capacity on in vitro fertilization.

ABSTRACT   서론   재료 및 방법    정액의 준비    Percoll을 이용한 정자 분리    정액 동결 과정    동결 정액 융해 및 생존율 평가    난자의 수집 및 체외성숙    체외수정 및 체외배양    실험설계    통계처리(Statistical Analysis)   결과    Percoll 분리 후 동결보존액 LEY와 Triladyl를 이용한 동결-융해 후의 미니돼지 정자의 생존율 비교 평가    체외수정 시 Percoll 분리된 LEY와 Triladyl를 이용한 동결정액이 수정율, 분할율 및 배반포 발달율에 미치는 영향   고찰   사사   인용문헌

• 이상희(강원대학교 동물생명과학대학) | Sang-Hee Lee
• 유한준(강원대학교 동물생명과학대학) | Han-Jun Yoo
• 이용승(강원대학교 동물생명과학대학) | Yong-Seung Lee
• 정희태(강원대학교 수의학부대학) | Hee-Tae Cheong
• 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang
• 김대영(가천의과대학교 생명과학부) | Dae-Young Kim
• 박춘근(강원대학교 동물생명과학대학) | Choon-Keun Park Corresponding author