The in vitro maturation rate of vitrified-thawed canine oocytes was 30.8±3.4%. The in vitro maturation rate of vitrified oocytes was lower than that of the control (52.0±2.5%, p<0.05). The in vitro maturation rate of vitrified-thawed oocytes were significantly (p<0.05) lower than those of fresh oocytes. The in vitro maturation and developmental rates of the vitrified-thawed oocytes were 17.5±2.5% and 8.8±3.4%, respectively. This results were lower than the control group (43.6±3.2% vs 20.0±3.0%). SOD1 gene expression of 1～2 mm of follilce size were higher than those of above 6 mm follicle size. SOD2 gene expression of 1～2 mm of follicle size were significantly higher than those of above 6 mm follicle size (p<0.01). The expression pattern of SOD1, 2 was constantly expressed in both groups but strongly expressed in follicles (1～2 mm) group when compared to the above 6 mm follicles. SOD gene expression between groups the fresh and vitrified oocytes groups were significant differences in rates. However, RGS gene expression between groups the fresh and vitrified oocytes groups were no significant differences in rates.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    In Vitro Maturation of Oocytes    Vitrification and Thawing of Oocytes    Gene Expression of Vitrified-Warmed Canine Oocytes    Statistical Analysis   RESULTS    Effects of Vitrification on In Vitro Maturation of CanineOocytes    In Vitro and Development of Vitrified-thawed Oocytes    SOD Gene Expression of Follicle Size    Gene Expression of Fresh and Vitrified Canine Oocytes   DISCUSSION   REFERENCES

• Ji-Hoon Park(College of Veterinary Medicine, Chungnam National University)
• Sang-Keun Kim(College of Veterinary Medicine, Chungnam National University) Corresponding author