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공여세포 처리 조건이 형질전환 복제돼지 생산에 미치는 영향

Effects of Donor Cell Treatments on the Production of Transgenic Cloned Piglets

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  • URLhttps://db.koreascholar.com/Article/Detail/190968
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

This study was conducted to investigate the effects of donor cell treatments on the production of transgenic cloned piglets. Ear fibroblast cell obtained from NIH MHC Inbred minipig was used as control. The GalT knock-out/CD46 knock-in (GalT/CD46) transgenic cell lines were established and used as donor cells. The reconstructed GalT/CD46 embryos were surgically transferred into oviduct of naturally cycling surrogate sows (Landrace × Yorkshire) on the second day of standing estrus. Unlike control (1.2 kV/cm,, 75.4%), the fusion rate of the GalT/CD46 donor cells was significantly higher in 1.5 kV/cm, (84.5%) than that of 1.25 kV/cm, (20.2%) (p<0.01). When the number of the transferred embryos were more than 129, the pregnancy and delivery rates were increased to 13/20 (65%) and 5/20 (25%) compared to less than 100 group [1/6 (16.7%) and 0/6 (0%)], respectively. To analyze the effect of donor cell culture condition on pregnancy and delivery rates, the GalT/CD46 donor cells were cultured with DMEM or serum reduced medium. In serum reduced medium group, the pregnancy and delivery rates were improved to 8/12 (66.7%) and 5/12 (41.7%) compared to DMEM group [3/7 (42.9%) and 0/7 (0%)], respectively. In conclusion, it can be postulated that an appropriate fusion condition and culture system is essential factors to increase the efficiency of the production of transgenic cloned piglets.

목차
ABSTRACT   서론   재료 및 방법    화학 조성물    미성숙 난자의 체외성숙    공여세포 준비    핵이식 과정    형질전환 복제란 이식 및 임신 확인    통계적 분석   결과    공여세포에 적합한 융합조건 확립    이식된 형질전환 복제란의 숫자가 임신율 및 분만율에 미치는 영향 분석    공여세포 배양조건에 따른 임신율 및 분만율 분석   고찰   인용문헌
저자
  • 권대진(농촌진흥청 국립축산과학원 동물바이오공학과) | Dae-Jin Kwon
  • 곽태욱(농촌진흥청 국립축산과학원 동물바이오공학과) | Tae-Uk Kwak
  • 오건봉(농촌진흥청 국립축산과학원 동물바이오공학과) | Keon Bong Oh
  • 김동훈(농촌진흥청 국립축산과학원 동물바이오공학과) | Dong Hoon Kim
  • 양병철(농촌진흥청 국립축산과학원 동물바이오공학과) | Byoung-Chul Yang
  • 임기순(농촌진흥청 국립축산과학원 동물바이오공학과) | Gi-Sun Im
  • 김진회(건국대학교 동물생명과학대학) | Jin-Hoi Kim
  • 박진기(농촌진흥청 국립축산과학원 동물바이오공학과) | Jin-Ki Park
  • 황성수(농촌진흥청 국립축산과학원 동물바이오공학과) | Seongsoo Hwang Corresponding author