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수퇘지 정자의 운동성, 생존성 및 체외수정 능력에 대한 시판 액상 정액

Effect of Storage in Different Commercial Semen Extenders on the Motility, Viability and Fertility In Vitro of Boar Spermatozoa

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  • URLhttps://db.koreascholar.com/Article/Detail/190969
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

The objective of this study was to determine the effect of semen extenders on the motility, viability and fertility in vitro of spermatozoa during storage of fresh boar semen diluted in different commercial extenders used for pig artificial insemination (AI). In this experiment, semen were diluted in Androhep plus, Beltsville Thawing Solution (BTS), Modena, Seminark and Vitasem LD. Five ejaculates were collected from three Duroc boars and sub-samples were diluted (30×106 spermatozoa/ml) in different extenders. Semen was stored at 17℃ for 10 days. Sperm motility and viability was assessed using Computer-Assisted Semen Analysis (CASA) and flow-cytometry on 1, 3, 5 and 10 day post collection. The motility of spermatozoa stored in different extenders was gradually decreased by increasing the duration of storage of semen. However, there was not significantly different in the sperm motility and viability among other extenders. On the other hand, the in vitro-matured oocytes were fertilized and cultured in vitro to assess the fertility of boar spermatozoa stored for 3 and 10 days in different extenders. The percentage of morula and blastocyst were taken as indicators of fertility in vitro of spermatozoa. Therefore, there were no differences in the rate of embryos developed to the molular and blastocyst stage. There were no differences in the motility and fertility in vitro among 5 kinds of commercial boar semen extenders.

목차
ABSTRACT   서론   재료 및 방법    시약 및 보존액    정액의 준비    희석정액의 운동성 및 생존성 평가    난자의 수집 및 체외성숙    체외수정 및 체외배양    실험설계    통계처리   결과   고찰   인용문헌
저자
  • 사수진(농촌진흥청 국립축산과학원 양돈과) | Soo Jin Sa
  • 김명직(농촌진흥청 국립축산과학원 양돈과) | Myung Jick Kim
  • 조규호(농촌진흥청 국립축산과학원 양돈과) | Kyu Ho Cho
  • 김두완(농촌진흥청 국립축산과학원 양돈과) | Du Wan Kim
  • 소경민(농촌진흥청 국립축산과학원 양돈과) | Kyoung Min So
  • 정기화(경남과학기술대학교 동물소재공학과) | Ki Hwa Chung
  • 손중호(노아바이오텍) | Jung Ho Son
  • 김인철(농촌진흥청 국립축산과학원 양돈과) | In Cheul Kim Corresponding author