The objective of this study was to evaluate efficiency of methyl-beta-cyclodextrin (MBCD) in the sperm preservation of bull. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 1, 5, 10 and 20 mM MBCD before freezing process. Analysis of viability in frozen-thawed sperm was estimated by SYBR14/PI double stain, hypoosmotic swelling test(HOST) and acrosome damage with FITC-PNA, and mitochondria activation with Rhodamin123 by flow-cytometry. The sperm viability was significantly higher in 0 mM and 5 mM concentrations of MBCD than other groups (p<0.05). However, the HOST was significantly lower at 20 mM concentration of MBCD than other concentrations (p<0.05). In addition, acrosome damage and mitochondria activation rates were significantly lower at 20 mM concentration of MBCD than other groups (p<0.05). In conclusion, the viability of sperm was not significantly different among concentrations of MBCD 0, 5 and 10 mM, but MBCD 20 mM was significantly lower than other groups. In addition, as concentrations of MBCD was high, HOST, acrosome damage and mitochondria activation rates had a negative effect in bull sperm.

ABSTRACT   서론   재료 및 방법    정액의 준비    정액동결    동결 정액 융해 및 성상 검사    생존율 검사(SYBR-14/PI Staining)    정자 원형질막 기능검사(Hypoosmotic Swelling Test)    첨체 손상율 검사(FITC-PNA/PI Staining)    미토콘드리아 기능율 검사(Rhodamin123/PI Staining)    통계처리   결과    동결 보존액 내 MBCD의 농도별 처리 후 정자의 생존율 비교 평가    정자의 원형질막 기능검사    동결 보존액 내 MBCD의 농도별 처리 후 정자의 첨체 손상검사    정자의 미토콘드리아 기능율 검사   고찰   인용문헌

• 이경진(강원대학교 동물생명과학대학) | Kyung-Jin Lee
• 서기범(강원대학교 동물생명과학대학) | Gi-Beom Seo
• 이용승(강원대학교 동물생명과학대학) | Yong-Seung Lee
• 유한준(강원대학교 동물생명과학대학) | Han-Jun Yoo
• 정희태(강원대학교 수의과대학) | Hee-Tae Cheong
• 이승환(국립축산과학원) | Seung-Hwan Lee
• 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang
• 박춘근(강원대학교 동물생명과학대학) | Choon-Keun Park Corresponding author