Acteoside (verbascoside) is a typical phenylethanoid glycoside, extracted from various plants. It has various biological functions such as anti-oxidant, anti-inflammation, and anti-hypertension. Specially, it was powerful anti-oxidants either by direct scavenging of reactive oxygen and nitrogen species, or by acting as chain-breaking peroxyl radical scavengers. We examined the role of acteoside in IVM medium on the morphological progress of meiosis, developmental competence, and ROS in porcine oocytes. And we investigated effect of acteoside on the oocytes condition represented by cytoplasmic maturation by homogeneous distribution and formation of cytoplasmic organelles and regulation of apoptosis-related genes. The selected COCs were cultured in TCM-199 with various concentration of acteoside: 0 (control), 10, 30, and 50 μM. After 22 h of maturation with hormones, the oocytes were washed twice in a fresh maturation medium before being cultured in hormone-free medium for additional 22 h. The oocytes maturation rates of supplemented with acteoside were no significantly different compared with control group (71.13, 75.96, 72.95 and 73.68%, respectively). Level of ROS was significantly decreased in acteoside treated group. Furthermore, the parthenogenetic blastocyst rate was significantly improved in 10 μM acteoside treated group compared with control group (40.03 vs. 22.95%). During IVM, 10 μM acteoside treated oocytes showed that the mitochondria and lipid droplet were smaller and homogeneous distribution in cytoplasm compare with non-treated control oocytes. And reverse transcription polymerase chain reaction (RT-PCR) witarthenogenetic blstocysts revealed that acteoside increased the anti-apoptoticgenes, otherwise reibued pro-apoptotic genes. In conclusion, our results represents that addition of acteoside to the IVM medium has a beneficial effect in physiology of porcine oocytes such as viability and activation, providing a improved method for porcine oocytes in vitro.

• K. J. Kim(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• J. H. Lee(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• X. X. Li(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• E. Y. Kim(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• Y. B. Park(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• K. S. Park(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• Y. N. Ha(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• M. Kim(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• K. W. Han(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• J. Y. Park(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• J. Yu(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• D. S. Lee(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)
• Min Kyu Kim(Lab. of Animal Reproduction and Physiology, Department of Animal Resource and Biotechnology, College of Agriculture Life Science, Chungnam National University)