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CDK9 Regulates Global Transcription in Growth Phase of Pig Oocytes
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/191587
In vitro production of porcine embryos, including in vitro maturation of oocytes followed by in vitro fertilization and in vitro culture, may result in live offspring, but it is still associated with great inefficiencies. In mammalian oocytes, acquisition of meiotic competence coincides with a decrease in general transcriptional activity at the end of the oocyte growth phase. In this study, we investigated the expression and sub‐cellular localization of CDK9, a RNA polymerase II CTD kinase during pig oocyte growth. Localization and expression of components involved in mRNA and rRNA transcription were assessed by immunocytochemistry in growing and fully‐grown oocytes. In addition, meiotic resumption, germinal vesicle breakdown and nuclear transcription were analyzed in oocytes cultured in presence of a potent CDK9 inhibitor, flavopiridol. Our analyses, demonstrated that CDK9 became co‐localized partially with phosphorylated Pol II CTD and mRNA splicing complexes. Surprisingly, CDK9 was co‐localized with Pol Ispecific transcription factor, UBF, and gradually localized in nucleolar peripheries at the final steps of oocyte growth. Treatment with flavopiridol resulted in arrest in meiotic resumption, germinal vesicle breakdown as well as a decline in global transcription. All together, this data suggest that CDK9 has a dual role in both Pol I‐ and Pol II‐dependent transcription in pig oocyte growth.
