The event that occur in sperm during chemotaxis are only partly known. As a essential step of fertilization, sperm cells should undergo capacitation process inside female genital tracts. To understand the molecular event of calcium signals on sperm cells, Fluo 4 loaded spermatozoa was treated with follicular fluid. The motility of sperm was reduced by follicular fluids. Simultaneously, level of calcium in head and tail was also reduced for 5 10 second. The inhibition of sperm motility was believed as a reversible event, so the follicular fluid in graffiaan follicles in vivo could act as a selector on active spermatozoa that recover motility and calcium signals during ovulation. This suggested that the normal levels of calcium in sperm was also critical for active state of sperm cells and the follicular fluids during ovulation could inhibit the motility of sperm cell via calcium signaling.

• Sung Woo Kim(Animal Genetic Resources Station, National Institute of Animal Science, RDA)
• In-Sul Hwang(Interdisciplinary Graduate School of Science and Technology, Shinshu)
• Hyun Kim(Animal Genetic Resources Station, National Institute of Animal Science, RDA)
• Jin-Ki Park(UniversityAnimal Biotechnology Division, National Institute of Animal Science, RDA)
• Soo-Bong Park(Bio-resources Management Division, Reserch Policy Bureau, RDA)
• Boh-Suk Yang(Animal Genetic Resources Station, National Institute of Animal Science, RDA)