The experimental manipulation of protooncogenes and their gene products is a valuable research tool for the study of human neoplasia. In this study, the recently identified human cervical cancer protooncogene (HccR-2) was expressed in transgenic mice under the control of the tetracycline regulatory system. The phenotype observed was similar in many respects to human chronic neutrophilic leukemia (CNL). Thus, the HccR-2 transgenic mouse model is important not only for investigating the biological properties of the HccR-2 protooncogene in vivo, but also for analyzing the mechanisms involved in the progression of CNL.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    Production of Transgenic Mice    PCR Analysis    Southern Blot Hybridization Analysis    RNA Analysis by Reverse Transcriptase-PCR (RT-PCR)    Western Blot Hybridization Analysis    Morphologic and Histologic Analyses    Statistical Analysis   RESULTS    Generation of the Transgenic Mice Expressing HccR-2    Peripheral Blood and Bone Marrow Features    Development of Chronic Neutrophilic Leukemia (CNL)    Leukemic Transgenic Mice were Susceptible to Infection   DISCUSSION   REFERENCES

• Byoung Boo Seo(Dept. of Animal Resources, College of Life & Environmental Science, Daegu University)
• Sung-Hun Min(Dept. of Biotechnology, College of Engineering, Daegu University)
• Eun Ji Lee(Dept. of Obstetrics and Gynecololgy, Yeung nam University)
• Zae Young Ryoo(School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University)
• Humdai Park(Dept. of Biotechnology, College of Engineering, Daegu University) Corresponding author