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Effect of Porcine Oocyte Chromatin Configuration on the Maturation and Development Potential

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/213348
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

In all the studies of mammalian species, chromatin in the germinal vesicle (GV) is initially decondensed with the nucleolus not surrounded by heterochromatin (the NSN configurations). During oocyte growth, the GV chromatin condenses into perinucleolar rings (the SN configurations) or other corresponding configurations with or without the perinucleolar rings, depending on species. During oocyte maturation, the GV chromatin is synchronized in a less condensed state before germinal vesicle breakdown (GVBD) in species that has been minutely studied. As not all the species show the SN configuration and gene transcription always stops at the late stage of oocyte growth, it is suggested that a thorough condensation of GV chromatin is essential for transcriptional repression. Because the GV chromatin status is highly correlated with oocyte competence, oocytes must end the NSN configuration before they gain the full meiotic competence and they must take on the SN or corresponding configurations to stop gene transcription before they acquire the competence for early embryonic development. In this study, we firstly investigated whether the follicle size could determine chromatin configuration in porcine oocyte. For this experiment, follicles was divided into three groups (<1 mm follicle, 1~3 mm follicle and 3~6 follicle). Using DAPI staining, the GV nucleolus and chromatin of porcine oocytes was classified into SN, SN-NSN and NSN configurations. MⅠ and M Ⅱ of three groups's Mature oocytes by staining was confirmed the configuration of chromatin. The maturation rate and parthenogenetic development potential were significant different between the SN and NSN configurations oocytes. These results indicated that chromatin changes in GV oocytes affect the development potential of porcine embryos.

저자
  • Dong-Il Jin(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University)
  • Reza K. Oqani(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University)
  • Yun-Fei Diao(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University)
  • Rong-Xun Han(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University)
  • Jin-Yu Zhang(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University)
  • Min-Gu Lee(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pig, Chungnam National University)