The present study was to assess the in vitro viability and sexing rate of bovine embryos. Blastocysts were harvested on day 7～9 day after insemination(in vitro and in vivo), and the sex of the embryos was examined using the LAMP method. Embryo cell biopsy was carried out in a 80 μl drop Ca2+, Mg2+ free D-PBS and, biopsied embryos viability were evaluated after more 12 h culture in IVMD culture medium. The formation of recovered embryo to expanded and hatching stages had ensued in higher of sexed embryo in vivo than in vitro (100% vs. 89%, p<0.05), and in vitro, the rates of degeneration after sexing were significantly (p<0.05) higher in vitro than in vivo(11% vs. 0.0%). The rates of the predicted sex were female 61% vs. 56%, and male 39% vs. 44% in vivo and in vitro, respectively. The rates of survival following different biopsy methods were seen between punching and bisection method in vivo and in vitro (100% vs. 89% and 100% vs, 78% respectively). Biopsy method by punching was significantly (p<0.05) higher than bisection between produced embryos in vivo and in vitro. The present data indicate that with microblade after punching for embryo sexing results in high incidence of survivability on development after embryo biopsy. It is also suggested that LAMP-based embryo sexing suitable for field applications.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    In Vitro Embryo Production    In Vivo Embryo Production    Embryo Biopsy    LAMP Analysis    Experimental Design    Statistical Analysis   RESULTS   DISCUSSION   REFERENCES

• Sang-Rae Cho(Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea) Corresponding author
• In-Cheol Cho(Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea)
• Nam-Young Kim(Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea)
• Sang-Hyun Han(Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea)
• Yong-Sang Park(Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea)
• Moon-Suck Ko(Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea)
• Chang-Young Choe(Dairy Science Division, National Livestock Institute, Rural Development Administration, Cheonan, 331-801, Korea)
• Jun-Kyu Son(Dairy Science Division, National Livestock Institute, Rural Development Administration, Cheonan, 331-801, Korea)
• Jae-Gyu Yoo(Animal Biotechnology Division, National Livestock Institute, Rural Development Administration, Suwon 441-706, Korea)
• Hyung-Jong Kim(Planning & Coordination, National Livestock Institute, Rural Development Administration, Suwon 441-706, Korea)
• Yeong-Gyu Ko(Division, Animal Genetic Resources Station, National Livestock Institute, Rural Development Administration, Namwon, 590-830, Korea)