LIVE/DEAD® BacLight™ and alamarBlue® are fluorescent materials used for the enumeration of live and dead bacteria. LIVE/DEAD® BacLight™ is generally used for confocal microscopy applications to differentiate live from dead bacteria in a biofilm or planktonic state. AlamarBlue® has also been used widely to assay live and dead bacteria in a planktonic state. Whilst these materials are successfully utilized in experiments to discriminate live from dead bacteria for several species of bacteria, the application of these techniques to oral bacteria is limited to the use of LIVE/ DEAD® BacLight™ in biofilm studies. In our present study, we assessed whether these two methods could enumerate live and dead oral bacterial species in a planktonic state. We tested the reagents on Streptococcus mutans, Streptococcus sobrinus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Enterococcus faecalis and found that only LIVE/DEAD® BacLight™ could differentiate live from dead cells for all five of these oral strains. AlamarBlue® was not effective in this regard for P. gingivalis or A. actinomycetemcomitans. In addition, the differentiation of live and dead bacterial cells by alamarBlue® could not be performed for concentrations lower than 2 × 106 cells/ml. Our data thus indicate that LIVE/DEAD® BacLight™ is a more effective reagent for this analysis.

ABSTRACT   1. 서론   2. 중국 웹보드게임산업 현황 분석    2.1 웹보드게임의 정의와 특징    2.2 웹보드게임산업 현황   3. 한국 웹보드게임 산업현황 분석    3.1 한국 웹보드게임 산업 규모    3.2 한국 웹보드게임 사용자 현황    3.3 대표적인 웹보드게임회사: 한게임    3.4 한국 웹보드게임의 산업 내 역할   4. 한-중 웹보드게임 산업의 비교    4.1 웹보드게임의 사회적 배경차이    4.2 웹보드게임 규제 규칙의 차이    4.3 웹보드게임 사용자 온라인접근제도의 차이   5. 결론 및 시사점   참고문헌

• 임호(중국화동이공대학교 법학원 부교수, 중국변호사) | Hu Ren Corresponding author