This study compares the abundance and community structure of zooplankton organisms from the littoral and pelagic regions, and considers particularly trophic levels vs. zooplankton abundances. Zooplankton samples, collected every 3 months over a year from 2010 to 2011 at 29 temperate lakes and reservoirs, which belong to two different river basins (Youngsan and Seomjin River). The spatial pattern of rotifers was similar to that of total zooplankton abundance. This reflected the fact that rotifers strongly dominated the zooplankton community. There were considerable spatial variations in total zooplankton abundance (ANOVA, p<0.01), while there were no significant differences both in littoral and pelagic regions in abundance of zooplankton (ANOVA, p=0.205). The mean abundance of zooplankton in eutrophic systems was much higher than that of mesotrophic systems, while significant difference in number of species and diversity index were not shown in both trophic systems.