This study was conducted to investigate the effect of biological membrane transfer modifier, lysophospholipd (LPLs) on the parameters from in vitro rumen simulated fermentation. Commercially available LPLs product (LipidolTM) was supplemented into experimental diets which consisted of orchard grass and concentrate diet (60:40) in different levels (0.1%, 0.3% and 0.5%). Then in vitro rumen simulated fermentation was performed. Although, a declining trend of pH was found in treatments, all pH values were detected in a range relevant to normal rumen fermentation. Gas production, ammonia nitrogen and total VFA production were greatly influenced by the supplementation of LPLs. All parameters were increased along with increased levels of LPLs in diet. As a result, 0.1% of LipidolTM is recommended based on the determined in vitro rumen fermentative parameters in this study.

• Sangbuem Cho(Animal Resources Research Center Kon-Kuk University) Two authors are equally contributed
• Dong Hyun Kim(Department of Animal Science, Chonbuk National University) Two authors are equally contributed
• Il Hwan Hwang(Pathway Intermediates, Ltd. UK, Eazybio Co. Ltd, Korea)
• Nag-Jin Choi(Department of Animal Science, Chonbuk National University) Corresponding author