This study was conducted among 120 different breed cows at selected areas of Sirajgonj district from March to July 2010 to compare the reproductive performance of crossbred and Desi cows at farmer’s level. The results showed that the average daily milk yield of Desi, Shahiwal × Desi, Friesian × Desi and Jersey × Desi cows was 2.3 ± 0.2, 4.9 ± 0.9, 6.0 ± 1.0 and 5.7 ± 0.9 liters, respectively. The milk yield of crossbred cows (5.5 ± 0.6 liters/day) was significantly (p<0.01) higher than Desi cows (2.3 ± 0.2 liters/day). The average age at puberty of Shahiwal × Desi, Friesian × Desi and Jersey × Desi was significantly (p<0.01) lower than that of Desi breed. The crossbred cows had significantly (p<0.01) lower pubertal age (20.4 ± 1.2) than Desi (25.9 ± 1.1). The age at first calving in Desi cows was significantly (p<0.01) higher (37.6 ± 1.1 months) than crossbred cows (31.2 ± 1.3 months). The average gestation length of Desi, Shahiwal × Desi, Friesian × Desi and Jersey × Desi was 289.9 ± 1.4, 285.0 ± 0.0, 285.0 ± 4.2 and 282.1 ± 2.4 days, respectively. It is suggested that the overall reproductive performance of Friesian × Desi, Jersey × Desi and Shahiwal × Desi cows were better than that of Desi cow.