조직배양묘인 Dtps. pulcherrima (sib.) × Dtps. ‘Kyotoprinty’ 를 이용하여 호접란 체세포 변이체의 형태적 유전 적 변이를 조사하였다. 잎과 꽃에서의 색깔과 형태 변이 도 다양하게 관찰되었다. 조직배양시에 발생하는 잎의 변이와 꽃의 변이중에서 대표적인 개체들 13개를 골라 정상적인 개체와 함께 total genomic DNA를 분리하고 RAPD 분석하여 변이체와 정상적인 개체간 DNA 밴드 차이가 나는지 살펴보았다. 그 결과 Operon primer OPO-06과 OPO-07를 이용한 PCR 분석에서 다형성이 관찰되었다. 잎의 변이를 일으킨 개체와 꽃의 변이를 야 기한 개체에서 각각 특이한 DNA Marker를 선발할 수 있었다.

Morphological and genetic variations in Phalaenopsis somaclones of Dtps. pulcherrima (sib.) × Dtps. ‘Kyotoprinty’ obtained from tissue culture were examined. A variety of leaf and flower variations in color and shape were also observed. Thirteen clones showing leaf and flower variations during tissue culture were used for a RAPD analysis to know that there is a difference in DNA bands between somaclones and normal plants. Operon primer OPO-06 and OPO-07 gave polymorphism from PCR analysis. Comparing with the normal plant using primer OPO-07, specific DNA bands were observed in leaf variations. Primer OPO-06 showed specific bands especially in flower variations.

• Ae Sil Na(Dept. of Horticulture, Division of Applied Life Science, Graduate School Gyeongsang National University) | 나애실
• Byoung Ryong Jeong(Dept. of Horticulture, Division of Applied Life Science, Graduate School Gyeongsang National University, Institute of Agriculture & Life Science, Gyeongsang National University) | 정병룡 Corresponding author
• Chul Gu Been(Gyeongsangnam-Do Agricultural Research & Extension Services, Floricultural Breeding Institute) | 빈철구
• Ki Byung Lim(School of Applied Biosciences, Kyungpook National University) | 임기병