Animal manure and manure compost produced from livestock farms are widely applied in crop lands. Leachate and runoff water from them are presumed to be one of the major sources of water quality deterioration in rural streams. Laboratory experiments were conducted to investigate water quality characteristics and loading of leachate obtained under rainfall simulation. Manure composts for the experiments were collected from beef cattle farms, dairy farms, and pig farms. Water quality parameters include SS, COD, TN, and TP. Most parameters of leachate from pig manure compost was higher than others. Both TN and TP concentrations were reflecting the composition of manure itself. It is recommended, therefore that the leachate from the manure composts should be controlled not to be discharged into streams.