PURPOSES: This study proposes a novel method based on microscopic simulation models to evaluate bicycle passing ways in mixed traffic flow conditions at signalized intersections. METHODS: Both operational efficiency and safety are taken into consideration in the evaluation. A widely used performance measure, delay, is used for evaluating the operational efficiency. Regarding the safety evaluation, surrogate safety measures (SSM) to represent traffic conflicts and the level of crash severity, DeltaS and Max.DeltaV, are applied in the proposed method. RESULTS: Extensive simulations and statistical tests show that an integrated bike-box way is identified as the best in terms of operational efficiency and safety. CONCLUSIONS: The proposed method and outcomes of this study will be valuable for bicycle traffic operations and facility design.